ABSTRACT
OBJECTIVE:To systematically review the efficacy and safety of Shenxian shengmai oral liquid in the treatment of slow arrhythmia,and provide evidence-based reference for clinical treatment. METHODS:Cochrane library,EMbase,PubMed, CJFD,CBM and VIP database were retrieved to collect the randomized controlled trails(RCT)of Shenxian shengmai oral liquid(tri-al group)vs.other Chinese medicines(control group)in the treatment of slow arrhythmia. After quality evaluation,the Meta-analysis was performed using Rev Man 5.14 software. RESULTS:A total of 13 RCTs were included,involving 1 023 patients. The results of Meta-analysis showed that the effective rate [RR=1.26,95%CI(1.18,1.35),P<0.001] and average heart rate [MD=5.80,95%CI (4.01,7.58),P<0.001] in test group were significantly higher than control group;there were no significant difference in the inci-dence of adverse reactions between test group and control group[OR=0.46,95%CI(0.17,1.22),P=0.12]. CONCLUSIONS:Shenx-ian shengmai oral liquid is effective with good safety in the treatment of slow arrhythmia. However,due to low quality of included studies,it remains to be further verified by high-quality and large-sample long-term RCT.
ABSTRACT
Potential target proteins binding to VirB4 of type Ⅳ secretion system were screened during Brucella infected bovine embryonic trophoblast cells .Brucella VirB4 genes were amplified by PCR with species-specific primers .Expression vector pGBKT7-virB4 was constructed and analysed by sequencing and restriction enzymes ,transforming to the yeast strain Y187 and testing self-activation and toxicity .The cells model and cDNA library of bovine embryonic trophoblast cells infected with Brucella abortus strain were constructed respectively .Utilizing yeast two-hybrid system was employed to screen the target proteins of bovine embryo trophoblastic cells which was conjunctive with virB4 .These proteins were detected by real-time fluo-rescence quantitative PCR .The results suggested that bait plasmid pGBKT7-virB4 was successfully transformed into the Y187 and there was no toxicity and self-activation;the cDNA library of bovine embryonic trophoblast cells infected with Brucella abortus strain was constructed .There screened 13 positive plasmids in which Q10 and SLC3A2 were up-regulated at the mRNA level .In this paper ,we reported the interactions between the VirB4 protein of Brucella and the bovine embryo trophoblastic cells ,which provide an upstream work for further elucidating the pathogenesis of Brucella infection of the host cell .
ABSTRACT
Objective To investigate the relationship of mental status,personality characterstics and TCM syndromes of spontaneous abortion(SA).Methods Symptom Checklist 90,Measure of Mental Health,Eysenck Personality Questionnaire and Diagnostic Questionnaire of TCM syndromes were adopted.Multifactorial analysis was fitted by non-conditional logistic regression model.Results In patients with SA,a positive correlation of somatization factors and kidney dificiency,spleen deficiency,blood heat and liver-qi stagnation.A positive correlation was also found between hostility factors and kidney deficiency,obsessive-compulsive factors and liver-qi stagnation.Patients with melancholic temperament tended to manifest as the syndromes of kidney deficiency,spleen deficiency and liver-qi stagnation,and choleric temperament tended to manifest as blood heat and liver-qi stagnation.Conclusion The syndromes of kidney deficiency,spleen deficiency,blood heat and liver-qi stagnation at least partially reflect the psychosomatic reaction or psychosomatic disorder induced by mental factors.TCM syndromes has a relationship with mental status and personality characteristics.The nature of their relationship needs to be studied further.